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Alpha-synuclein oligomers are thought to be toxic mediators of Parkinson's disease and other alpha-synucleinopathies, but their histological detection in situ in diseased brain has been a challenge in the field for some time. Here we describe a method, the alpha-synuclein proximity ligation assay (AS-PLA), to detect alpha-synuclein oligomers in paraffin-embedded brain sections. Using AS-PLA previously unobserved alpha-synuclein oligomeric pathology is revealed.

Original publication

DOI

10.1007/978-1-4939-9124-2_7

Type

Journal article

Journal

Methods Mol Biol

Publication Date

2019

Volume

1948

Pages

69 - 76

Keywords

Aggregates, Alpha-synuclein, Oligomers, Parkinson’s disease, Pathology, Proximity ligation assay, Synucleinopathy