Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

Understanding the causal relationship between neural activity and behavior requires the ability to perform rapid and targeted interventions in ongoing activity. Here we describe a closed-loop all-optical strategy for dynamically controlling neuronal activity patterns in awake mice. We rapidly tailored and delivered two-photon optogenetic stimulation based on online readout of activity using simultaneous two-photon imaging, thus enabling the manipulation of neural circuit activity 'on the fly' during behavior.

Original publication

DOI

10.1038/s41592-018-0183-z

Type

Journal article

Journal

Nat Methods

Publication Date

12/2018

Volume

15

Pages

1037 - 1040

Keywords

Animals, Electric Stimulation, Female, Mice, Mice, Inbred C57BL, Nerve Net, Neurons, Optical Imaging, Optogenetics, Signal Processing, Computer-Assisted