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Understanding the causal relationship between neural activity and behavior requires the ability to perform rapid and targeted interventions in ongoing activity. Here we describe a closed-loop all-optical strategy for dynamically controlling neuronal activity patterns in awake mice. We rapidly tailored and delivered two-photon optogenetic stimulation based on online readout of activity using simultaneous two-photon imaging, thus enabling the manipulation of neural circuit activity 'on the fly' during behavior.

Original publication

DOI

10.1038/s41592-018-0183-z

Type

Journal article

Journal

Nat Methods

Publication Date

12/2018

Volume

15

Pages

1037 - 1040

Keywords

Animals, Electric Stimulation, Female, Mice, Mice, Inbred C57BL, Nerve Net, Neurons, Optical Imaging, Optogenetics, Signal Processing, Computer-Assisted