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The field of vascular biology has gained enormous insight from the use of Cre and inducible Cre mouse models to temporally and spatially manipulate gene expression within the endothelium. Models are available to constitutively or inducibly modulate gene expression in all or a specified subset of endothelial cells. However, caution should be applied to both the selection of allele and the analysis of resultant phenotype: many similarly named Cre models have divergent activity patterns while ectopic or inconsistent Cre or inducible Cre expression can dramatically affect results. In an effort to disambiguate previous data and to provide a resource to aid appropriate experimental design, here we summarize what is known about Cre recombinase activity in the most widely used endothelial-specific Cre and Cre/ERT2 mouse models.

Original publication

DOI

10.1161/ATVBAHA.118.309669

Type

Journal article

Journal

Arterioscler Thromb Vasc Biol

Publication Date

11/2018

Volume

38

Pages

2550 - 2561

Keywords

endothelial cells, gene expression, mice, phenotype, recombinases, Animals, Cardiovascular Diseases, Disease Models, Animal, Endothelial Cells, Gene Expression Regulation, Gene Targeting, Genotype, Integrases, Mice, Transgenic, Phenotype, Protein Interaction Domains and Motifs, Receptors, Estrogen, Selective Estrogen Receptor Modulators, Tamoxifen