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Inosine monophosphate dehydrogenase (IMPDH) and cytidine triphosphate synthase (CTPS) are two metabolic enzymes that perform rate-limiting steps in the de novo synthesis of purine and pyrimidine nucleotides, respectively. It has been shown that IMPDH and CTPS can comprise a filamentous macrostructure termed the cytoophidium, which may play a role in regulation of their catalytic activity. Although these two proteins may colocalise in the same cytoophidium, how they associate with one another is still elusive. As reported herein, we established a model HeLa cell line coexpressing OFP-tagged IMPDH2 and GFP-tagged CTPS1 and recorded the assembly, disassembly and movement of the cytoophidium in live cells. Moreover, by using super-resolution confocal imaging, we demonstrate how IMPDH- and CTPS-based filaments are aligned or intertwined in the mixed cytoophidium. Collectively, our findings provide a panorama of cytoophidium dynamics and suggest that IMPDH and CTPS cytoophidia may coordinate by interfilament interaction.

Original publication

DOI

10.1111/febs.14624

Type

Journal article

Journal

FEBS J

Publication Date

10/2018

Volume

285

Pages

3753 - 3768

Keywords

CTP synthase, IMP dehydrogenase, cytoophidium, live-cell imaging, super-resolution imaging, Carbon-Nitrogen Ligases, Cytidine Triphosphate, Cytoskeleton, Genes, Reporter, HeLa Cells, Humans, IMP Dehydrogenase, Microscopy, Confocal