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We demonstrate a two-photon optogenetic method that generates action potentials in neurons with single-cell precision, using the red-shifted opsin C1V1(T). We applied the method to optically map synaptic circuits in mouse neocortical brain slices and to activate small dendritic regions and individual spines. Using a spatial light modulator, we split the laser beam onto several neurons and performed simultaneous optogenetic activation of selected neurons in three dimensions.

Original publication

DOI

10.1038/nmeth.2249

Type

Journal article

Journal

Nat Methods

Publication Date

12/2012

Volume

9

Pages

1202 - 1205

Keywords

Action Potentials, Animals, Dendritic Spines, Mice, Neurons, Opsins, Optogenetics, Patch-Clamp Techniques, Photons