Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we will assume that you are happy to receive all cookies and you will not see this message again. Click 'Find out more' for information on how to change your cookie settings.
Skip to main content

While DNA vaccination using plasmid vectors is highly attractive, there is a need for further vector optimization regarding safety, stability, and efficiency. In this commentary, we review the minicircle vector (MC), which is an entity devoid of plasmid bacterial sequences, as an alternative to the traditional plasmid construct. The commentary highlights the recent discovery by Stenler et al. (2014) that the small size of an MC enables improved resistance to the shearing forces associated with e.g. pneumatic delivery methods. This observation may have implications for the regulatory agencies’ requirement of plasmid integrity and quality.

Original publication




Journal article


Human Vaccines and Immunotherapeutics


Taylor & Francis: STM, Behavioural Science and Public Health Titles

Publication Date



1306 - 1308


DNA vaccination, non-viral gene therapy, pneumatic delivery, supercoil, topoisoforms, vaccine, vector safety