Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

We describe a new approach for identifying and studying genes involved in Drosophila development. Single copies of an enhancer detector transposon, P[1ArB], have been introduced into flies at many different genomic locations. The beta-galactosidase reporter gene in this construct is influenced by a wide range of genomic transcriptional regulatory elements in its vicinity. Our results suggest that a significant proportion of these regulatory sequences are control elements of nearby Drosophila genes. These genes need not be disrupted for their regulatory elements to be identified by P[1ArB]. The P[1ArB] transposon has been designed to facilitate both rapid cloning and deletion analysis of genomic sequences into which it inserts. Therefore, the enhancer detection system is an efficient method of screening for genes primarily on the basis of their expression pattern and then rapidly analyzing those of particular interest at the molecular and genetic levels.

Original publication

DOI

10.1101/gad.3.9.1301

Type

Journal article

Journal

Genes Dev

Publication Date

09/1989

Volume

3

Pages

1301 - 1313

Keywords

Animals, DNA Transposable Elements, Drosophila melanogaster, Embryo, Nonmammalian, Embryonic and Fetal Development, Gene Expression Regulation, Genetic Vectors, Recombinant Fusion Proteins, Regulatory Sequences, Nucleic Acid, beta-Galactosidase