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The murine monoclonal antibody (MoAb) CB21, raised after immunization with sonicated extracts of human platelets, has been shown to react with a line-restricted surface molecule and also a cytoplasmic structure displaying no restriction in terms of lineage and species. The surface structure recognized by the CB21 MoAb is exclusively expressed on the surface membrane of human platelets, being undetectable on other cells or lines so far tested. After permeabilization, the majority of the cells and lines tested with the CB21 MoAb displayed strong cytoplasmic reactivity with a constant typical filamentous distribution. Biochemical and morphological analyses showed that the cytoplasmic counterpart recognized by the CB21 MoAb is the intermediate filament type III.

Original publication

DOI

10.1111/j.1365-3083.1990.tb02812.x

Type

Journal article

Journal

Scand J Immunol

Publication Date

05/1990

Volume

31

Pages

609 - 617

Keywords

Animals, Antibodies, Monoclonal, Blood Platelets, Cell Line, Cell Membrane, Colchicine, Cytoskeleton, Endothelium, Epitopes, Female, Guinea Pigs, Humans, Intermediate Filaments, Leukocytes, Mononuclear, Mice, Mice, Inbred BALB C, Muscles