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We describe an optical microscope system whose focal setting can be changed quickly without moving the objective lens or specimen. Using this system, diffraction limited images can be acquired from a wide range of focal settings without introducing optical aberrations that degrade image quality. We combine this system with a real time Nipkow disc based confocal microscope so as to permit the acquisition of extended depth of field images directly in a single frame of the CCD camera. We also demonstrate a simple modification that enables extended depth of field images to be acquired from different angles of perspective, where the angle can be changed over a continuous range by the user in real-time.

Original publication

DOI

10.1364/oe.16.021843

Type

Journal article

Journal

Opt Express

Publication Date

22/12/2008

Volume

16

Pages

21843 - 21848

Keywords

Algorithms, Animals, Equipment Design, Image Enhancement, Image Processing, Computer-Assisted, Imaging, Three-Dimensional, Mice, Microscopy, Microscopy, Confocal, Microscopy, Fluorescence, Optical Devices, Optical Fibers, Video Recording