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We describe a method of optical refocusing for high numerical aperture (NA) systems that is particularly relevant for confocal and multiphoton microscopy. This method avoids the spherical aberration that is common to other optical refocusing systems. We show that aberration-free images can be obtained over an axial scan range of 70 mum for a 1.4 NA objective lens. As refocusing is implemented remotely from the specimen, this method will enable high axial scan speeds without mechanical interference between the objective lens and the specimen.

Type

Journal article

Journal

Opt Lett

Publication Date

15/07/2007

Volume

32

Pages

2007 - 2009

Keywords

Algorithms, Animals, Equipment Design, Image Interpretation, Computer-Assisted, Kidney, Mice, Microscopy, Confocal, Microscopy, Fluorescence, Models, Statistical, Optics and Photonics, Photons, Reproducibility of Results