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We present a new method for setting a coverglass correction collar on an objective lens. Axial scans across the interface between the specimen volume and the slide are used together with a quantitative function of merit to determine the optimum setting of the correction collar. The method, which simplifies the adjustment for the user and reduces photobleaching, was implemented within the software environment of a scanning microscope system.

Original publication

DOI

10.1111/j.1365-2818.2005.01431.x

Type

Journal article

Journal

J Microsc

Publication Date

03/2005

Volume

217

Pages

184 - 187