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The Caenorhabditis elegans gene rec-1 was the first genetic locus identified in metazoa to affect the distribution of meiotic crossovers along the chromosome. We report that rec-1 encodes a distant paralog of HIM-5, which was discovered by whole-genome sequencing and confirmed by multiple genome-edited alleles. REC-1 is phosphorylated by cyclin-dependent kinase (CDK) in vitro, and mutation of the CDK consensus sites in REC-1 compromises meiotic crossover distribution in vivo. Unexpectedly, rec-1; him-5 double mutants are synthetic-lethal due to a defect in meiotic double-strand break formation. Thus, we uncovered an unexpected robustness to meiotic DSB formation and crossover positioning that is executed by HIM-5 and REC-1 and regulated by phosphorylation.

Original publication

DOI

10.1101/gad.266056.115

Type

Journal article

Journal

Genes Dev

Publication Date

15/09/2015

Volume

29

Pages

1969 - 1979

Keywords

HIM-5, REC-1, cyclin-dependent kinase, meiosis, meiotic crossover distribution, Animals, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Cell Cycle Proteins, Crossing Over, Genetic, DNA Breaks, Double-Stranded, Meiosis