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Muscle is an important and attractive target for gene therapy. Recent findings have shown that neutral amphiphilic triblock copolymers with a PEO-PPO-PEO arrangement significantly increase muscle transfection as compared to naked DNA. We were interested in evaluating whether reverse Pluronics (PPO-PEO-PPO) also possess transfection properties. Therefore, we measured the in vitro and in vivo transfection activity of 25R2 and 25R4, two copolymers that differ by their hydrophilic/hydrophobic balance. The results show that 25R2 significantly increases the transfection level in muscle compared to naked DNA. Taken together, this work demonstrates that the reverse Pluronic 25R2 possesses interesting properties for in vivo transfection.

Original publication

DOI

10.1002/mabi.201000463

Type

Journal article

Journal

Macromol Biosci

Publication Date

12/05/2011

Volume

11

Pages

590 - 594

Keywords

Animals, DNA, Electrophoretic Mobility Shift Assay, Enhancer Elements, Genetic, Female, Genes, Reporter, HEK293 Cells, Hep G2 Cells, Humans, Luciferases, Firefly, Mice, Mice, Inbred BALB C, Muscle, Skeletal, Poloxamer, Polyethylene Glycols, Propylene Glycols, Surface-Active Agents, Transfection