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The simultaneous sequencing of a single cell's genome and transcriptome offers a powerful means to dissect genetic variation and its effect on gene expression. Here we describe G&T-seq, a method for separating and sequencing genomic DNA and full-length mRNA from single cells. By applying G&T-seq to over 220 single cells from mice and humans, we discovered cellular properties that could not be inferred from DNA or RNA sequencing alone.

Original publication

DOI

10.1038/nmeth.3370

Type

Journal article

Journal

Nat Methods

Publication Date

06/2015

Volume

12

Pages

519 - 522

Keywords

Animals, Cell Line, Tumor, DNA, Genomics, Humans, Mice, Nucleic Acid Amplification Techniques, RNA, Messenger