G&T-seq: parallel sequencing of single-cell genomes and transcriptomes.
Macaulay IC., Haerty W., Kumar P., Li YI., Hu TX., Teng MJ., Goolam M., Saurat N., Coupland P., Shirley LM., Smith M., Van der Aa N., Banerjee R., Ellis PD., Quail MA., Swerdlow HP., Zernicka-Goetz M., Livesey FJ., Ponting CP., Voet T.
The simultaneous sequencing of a single cell's genome and transcriptome offers a powerful means to dissect genetic variation and its effect on gene expression. Here we describe G&T-seq, a method for separating and sequencing genomic DNA and full-length mRNA from single cells. By applying G&T-seq to over 220 single cells from mice and humans, we discovered cellular properties that could not be inferred from DNA or RNA sequencing alone.