Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we will assume that you are happy to receive all cookies and you will not see this message again. Click 'Find out more' for information on how to change your cookie settings.

We compared quantitative RT-PCR (qRT-PCR), RNA-seq and capture sequencing (CaptureSeq) in terms of their ability to assemble and quantify long noncoding RNAs and novel coding exons across 20 human tissues. CaptureSeq was superior for the detection and quantification of genes with low expression, showed little technical variation and accurately measured differential expression. This approach expands and refines previous annotations and simultaneously generates an expression atlas.

Original publication

DOI

10.1038/nmeth.3321

Type

Journal article

Journal

Nat Methods

Publication Date

04/2015

Volume

12

Pages

339 - 342

Keywords

Gene Expression Profiling, Humans, K562 Cells, Polymerase Chain Reaction, RNA, RNA, Long Noncoding, Sequence Analysis