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© 2014 Published by Elsevier Inc.Genome engineering has revolutionised genetic analysis in many organisms. Here we describe a simple and efficient technique to generate and detect novel mutations in desired target genes in Drosophila melanogaster. We target double strand breaks to specific sites within the genome by injecting mRNA encoding the Cas9 endonuclease and in vitro transcribed synthetic guide RNA into Drosophila embryos. The small insertion and deletion mutations that result from inefficient non-homologous end joining at this site are detected by high resolution melt analysis of whole flies and individual wings, allowing stable lines to be made within 1 month.

Original publication

DOI

10.1016/j.ymeth.2014.02.019

Type

Journal article

Journal

Methods

Publication Date

01/01/2014

Volume

69

Pages

128 - 136