The western painted turtle genome, a model for the evolution of extreme physiological adaptations in a slowly evolving lineage.
Shaffer HB., Minx P., Warren DE., Shedlock AM., Thomson RC., Valenzuela N., Abramyan J., Amemiya CT., Badenhorst D., Biggar KK., Borchert GM., Botka CW., Bowden RM., Braun EL., Bronikowski AM., Bruneau BG., Buck LT., Capel B., Castoe TA., Czerwinski M., Delehaunty KD., Edwards SV., Fronick CC., Fujita MK., Fulton L., Graves TA., Green RE., Haerty W., Hariharan R., Hernandez O., Hillier LW., Holloway AK., Janes D., Janzen FJ., Kandoth C., Kong L., de Koning AP., Li Y., Literman R., McGaugh SE., Mork L., O'Laughlin M., Paitz RT., Pollock DD., Ponting CP., Radhakrishnan S., Raney BJ., Richman JM., St John J., Schwartz T., Sethuraman A., Spinks PQ., Storey KB., Thane N., Vinar T., Zimmerman LM., Warren WC., Mardis ER., Wilson RK.
BACKGROUND: We describe the genome of the western painted turtle, Chrysemys picta bellii, one of the most widespread, abundant, and well-studied turtles. We place the genome into a comparative evolutionary context, and focus on genomic features associated with tooth loss, immune function, longevity, sex differentiation and determination, and the species' physiological capacities to withstand extreme anoxia and tissue freezing. RESULTS: Our phylogenetic analyses confirm that turtles are the sister group to living archosaurs, and demonstrate an extraordinarily slow rate of sequence evolution in the painted turtle. The ability of the painted turtle to withstand complete anoxia and partial freezing appears to be associated with common vertebrate gene networks, and we identify candidate genes for future functional analyses. Tooth loss shares a common pattern of pseudogenization and degradation of tooth-specific genes with birds, although the rate of accumulation of mutations is much slower in the painted turtle. Genes associated with sex differentiation generally reflect phylogeny rather than convergence in sex determination functionality. Among gene families that demonstrate exceptional expansions or show signatures of strong natural selection, immune function and musculoskeletal patterning genes are consistently over-represented. CONCLUSIONS: Our comparative genomic analyses indicate that common vertebrate regulatory networks, some of which have analogs in human diseases, are often involved in the western painted turtle's extraordinary physiological capacities. As these regulatory pathways are analyzed at the functional level, the painted turtle may offer important insights into the management of a number of human health disorders.