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The methods for evaluating internalization pathways of cellular CPP-mediated ON delivery utilizing a pre-mRNA splice correction assay and fluorescence-based quantification are described. Examples for characterization of CPP uptake routes, employing various endocytosis inhibitors, and special treatment conditions are demonstrated. The methods are developed to characterize cellular delivery of pre-mRNA splice switching peptide nucleic acids conjugated to CPPs by disulfide bond.

Original publication

DOI

10.1007/978-1-60761-919-2_16

Type

Chapter

Publication Date

2011

Volume

683

Pages

219 - 230

Keywords

Amino Acid Sequence, Base Sequence, Cell-Penetrating Peptides, Disulfides, Drug Carriers, Endocytosis, Fluorometry, HeLa Cells, Humans, Luciferases, Molecular Sequence Data, Oligonucleotides, Peptide Nucleic Acids, Protein Transport, RNA Splicing, Transfection