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Novel ubiquitin-based active site probes including a fluorescent tag have been developed and evaluated. A new, functionalizable electrophilic trap is utilized allowing for late stage diversification of the probe. Attachment of fluorescent dyes allowed direct detection of endogenous deubiquitinating enzyme (DUB) activities in cell extracts by in-gel fluorescence imaging.

Original publication

DOI

10.1039/c2ob25258a

Type

Journal article

Journal

Org Biomol Chem

Publication Date

07/05/2012

Volume

10

Pages

3379 - 3383

Keywords

Catalytic Domain, Click Chemistry, Endopeptidases, Enzyme Assays, Fluorescent Dyes, HEK293 Cells, Humans, Models, Molecular, Ubiquitin