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MicroRNAs are a group of small non-coding RNAs approximately 22 nucleotides in length. Recent work has shown differential expression of mature microRNAs in human cancers. We characterized the alteration in expression of a select group of microRNAs in primary peritoneal carcinoma relative to matched cases of ovarian serous carcinoma. MicroRNA expression was analysed using semi-quantitative stem-loop RT-PCR on a set of 34 formalin-fixed paraffin-embedded samples. Protein expression of p53 and bcl-2 was quantified in the corresponding tissue microarray. We provide definitive evidence that there is downregulation of a select group of microRNAs in tumours meeting Gynaecological Oncology Group criteria for primary peritoneal carcinoma relative to ovarian serous carcinoma. Specifically, we show decreased p53 expression and downregulation of miR-195 and miR-497 from the microRNA cluster site at chromosome 17p13.1 in primary peritoneal carcinoma relative to ovarian serous carcinoma. miR-195 and miR-497 may have potential roles as tumour-suppressor genes in primary peritoneal tumourigenesis.

Original publication

DOI

10.1038/modpathol.2008.135

Type

Journal article

Journal

Mod Pathol

Publication Date

02/2009

Volume

22

Pages

197 - 205

Keywords

Adult, Aged, Aged, 80 and over, Carcinoma, Chromosomes, Human, Pair 17, Down-Regulation, Female, Gene Expression Regulation, Neoplastic, Humans, Immunohistochemistry, MicroRNAs, Middle Aged, Ovarian Neoplasms, Peritoneal Neoplasms, Proto-Oncogene Proteins c-bcl-2, Reverse Transcriptase Polymerase Chain Reaction, Tissue Array Analysis, Tumor Suppressor Protein p53