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The MLL gene is frequently involved in chromosomal translocations associated with high-risk acute leukaemia. Infant and therapy-related acute leukaemia patients display chromosomal breakpoints preferentially clustered in the telomeric portion of the MLL breakpoint cluster region (SCII). Here, we demonstrate that SCII colocalizes with a gene-internal promoter element in the mouse and human MLL gene, respectively. The mRNA generated encodes an N-terminally truncated version of MLL that still exhibits many functional regions, including the C-terminal SET-domain. Etoposide-induced DNA double-strand breaks colocalize with the binding site of RNA polymerase II and the transcription initiation region, but not with a nearby Topo II consensus sequence. Thus, the observed genomic instability of the human MLL gene is presumably linked to transcriptional processes. The consequences of this novel finding for the creation of chromosomal translocations, the biology of the MLL protein and for MLL-mediated acute leukaemia are discussed.

Original publication

DOI

10.1038/sj.onc.1209948

Type

Journal article

Journal

Oncogene

Publication Date

01/03/2007

Volume

26

Pages

1361 - 1371

Keywords

Animals, Chromatin, Etoposide, Histone-Lysine N-Methyltransferase, Humans, Mice, Myeloid-Lymphoid Leukemia Protein, Promoter Regions, Genetic, RNA Polymerase II, Recombination, Genetic, Transcription, Genetic