A multi-color flow cytometric method for characterizing murine reticulated platelets using SYTO 13.
Hamad MA., Krauel K., Schanze N., Zurek M., Olivier CB., Kohl P., Nührenberg TG., Duerschmied D.
Reticulated platelets (RP) are immature platelets with heightened RNA content. An increased level of RP in the circulation is associated with various pathological conditions. In this study, we employed a novel flow cytometry approach for RP detection in mice, utilizing the nucleic acid dye SYTO 13 in conjunction with a platelet-specific marker (anti-mouse CD42b-DyLight 649). The efficacy of SYTO 13 for RP identification was confirmed by higher circulating RP levels in platelet-depleted mice during the recovery phase (35% ± 13%) compared to untreated mice (11% ± 1%, n = 9, p