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Fast synaptic neurotransmission in the vertebrate central nervous system relies primarily on ionotropic glutamate receptors (iGluRs), which drive neuronal excitation, and type A γ-aminobutyric acid receptors (GABAARs), which are responsible for neuronal inhibition. However, the GluD1 receptor, an iGluR family member, is present at both excitatory and inhibitory synapses. Whether and how GluD1 activation may affect inhibitory neurotransmission is unknown. In this work, by using a combination of biochemical, structural, and functional analyses, we demonstrate that GluD1 binds GABA, a previously unknown feature of iGluRs. GluD1 activation produces long-lasting enhancement of GABAergic synaptic currents in the adult mouse hippocampus through a non-ionotropic mechanism that is dependent on trans-synaptic anchoring. The identification of GluD1 as a GABA receptor that controls inhibitory synaptic plasticity challenges the classical dichotomy between glutamatergic and GABAergic receptors.

Original publication

DOI

10.1126/science.adf3406

Type

Journal article

Journal

Science

Publication Date

22/12/2023

Volume

382

Pages

1389 - 1394

Keywords

Animals, Mice, gamma-Aminobutyric Acid, Glutamate Dehydrogenase, Hippocampus, Neuronal Plasticity, Receptors, GABA, Synapses, Synaptic Transmission, Neural Inhibition, Mice, Knockout, Racemases and Epimerases