Inhibitory GABAergic interneurons of the mouse neocortex are a highly heterogeneous population of neurons that originate from the ventral telencephalon and migrate tangentially up into the developing cortical plate. The majority of cortical interneurons arise from a transient embryonic structure known as the medial ganglionic eminence (MGE), but how the remarkable diversity is specified in this region is not known. We have taken a genetic fate mapping strategy to elucidate the temporal origins of cortical interneuron subtypes within the MGE. We used an inducible form of Cre under the regulation of Olig2, a basic helix-loop-helix transcription factor highly expressed in neural progenitors of the MGE. We observe that the physiological subtypes of cortical interneurons are, to a large degree, unique to their time point of generation.
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Action Potentials, Age Factors, Animals, Animals, Newborn, Basic Helix-Loop-Helix Transcription Factors, Body Patterning, Cell Differentiation, Cell Movement, Cerebral Cortex, Embryo, Mammalian, Gene Expression Regulation, Developmental, Green Fluorescent Proteins, In Vitro Techniques, Interneurons, Mice, Mice, Transgenic, Nerve Tissue Proteins, Oligodendrocyte Transcription Factor 2, Patch-Clamp Techniques, Stem Cells, Telencephalon