Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

We demonstrate a two-photon optogenetic method that generates action potentials in neurons with single-cell precision, using the red-shifted opsin C1V1(T). We applied the method to optically map synaptic circuits in mouse neocortical brain slices and to activate small dendritic regions and individual spines. Using a spatial light modulator, we split the laser beam onto several neurons and performed simultaneous optogenetic activation of selected neurons in three dimensions.

Original publication

DOI

10.1038/nmeth.2249

Type

Journal article

Journal

Nat Methods

Publication Date

12/2012

Volume

9

Pages

1202 - 1205

Keywords

Action Potentials, Animals, Dendritic Spines, Mice, Neurons, Opsins, Optogenetics, Patch-Clamp Techniques, Photons