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RNA interference (RNAi) has tremendous potential for specific silencing of disease-causing genes. Its clinical usage however critically depends on the development of carrier systems that can transport the RNAi-mediating small interfering RNA (siRNA) molecules to the cytosol of target cells. Recent reports have suggested that extracellular vesicles (EVs) form a natural transport system through which biomolecules, including RNA, is exchanged between cells. Therefore, EVs are increasingly being considered as potential therapeutic siRNA delivery systems.In this chapter we describe a method for preparing siRNA-loaded EVs, including a robust, scalable method to isolate them from cell culture supernatants.

Original publication

DOI

10.1007/978-1-4939-6728-5_14

Type

Journal article

Journal

Methods Mol Biol

Publication Date

2017

Volume

1545

Pages

197 - 204

Keywords

Drug delivery, Exosomes, Extracellular vesicles, Microvesicles, Size-exclusion chromatography, siRNA, Cell Fractionation, Cell Line, Extracellular Vesicles, Humans, Polymerase Chain Reaction, RNA, Small Interfering