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Adaptive optics is implemented in a harmonic generation microscope using a wavefront sensorless correction scheme. Both the second- and third-harmonic intensity signals are used as the optimization metric. Aberration correction is performed to compensate both system- and specimen-induced aberrations by using an efficient optimization routine based upon Zernike polynomial modes. Images of live mouse embryos show an improved signal level and resolution.

Original publication

DOI

10.1364/OL.34.003154

Type

Journal article

Journal

Opt Lett

Publication Date

15/10/2009

Volume

34

Pages

3154 - 3156

Keywords

Air, Animals, Cytoplasm, Developmental Biology, Embryo, Mammalian, Equipment Design, Glass, Image Processing, Computer-Assisted, Mice, Microscopy, Confocal, Models, Statistical, Optics and Photonics, Refractometry, Time Factors