Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

During kidney development, the ureteric bud (UB), an outgrowth from the nephric duct, elongates and branches repeatedly to give rise to the renal collecting system. This process is stimulated by  GDNF  signaling via Ret receptor tyrosine kinase and transcription factors Etv4 and Etv5. Inducible genetic fate mapping showed that UB tip cells, which specifically express Ret and Etv4/Etv5, are progenitors for the growing collecting ducts. To investigate how the behaviors of these cells are influenced by Ret – Etv4/5 signaling, we first performed time-­‐lapse imaging of genetically mosaic organ cultures. These studies revealed that Ret signaling, via Etv4 and Etv5, promotes competitive cell rearrangements, which cause a subset of cells in the nephric duct to form the first UB tip. To ask whether similar cell rearrangements contribute to UB branching, we performed clonal analyses, using several methods to induce fluorescently labeled, recombinant clones in which the gene dosage of Ret or Etv4 was altered. Analysis of such clones, in time-­‐lapse movies of developing kidneys, indicate that the Ret – Etv4/5 pathway controls epithelial cell movements, which maintain the tip (progenitor) cell population and may promote epithelial branching.

Hosts: Prof Shankar Srinivas (Department of Physiology, Anatomy & Genetics)

Organisers: Prof Shankar Srinivas (Department of Physiology, Anatomy & Genetics)

Contact email: shankar.srinivas@dpag.ox.ac.uk