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The scanning two-photon fluorescence microscope produces optically sectioned images from the focal plane. It is sometimes desirable to acquire images from other planes of the specimen that are inclined with respect to the focal plane. In this Letter, we discuss the issues concerned with acquiring such images together with the effects of the inclination angle on image resolution and sectioning strength. To obtain images from oblique planes at high speed, a two-photon system was built wherein a novel optical system is used to provide aberration-free scanning.

Original publication

DOI

10.1364/OL.36.000663

Type

Journal article

Journal

Opt Lett

Publication Date

01/03/2011

Volume

36

Pages

663 - 665

Keywords

Microscopy, Photons, Pollen, Rotation